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The chickpea (Cicer arieitnum L.) is an important food legume crop of the family Fabaceae with high protein levels that is widely grown in rainfed areas prone to drought stress. It is a self-pollinated cool season crop with a true diploid... more
The chickpea (Cicer arieitnum L.) is an important food legume crop of the family Fabaceae with high protein levels that is widely grown in rainfed areas prone to drought stress. It is a self-pollinated cool season crop with a true diploid (2n = 16) nature. It is relatively cheap and a high source of protein. About 90% of the chickpea crop is grown by the use of residual moisture in the soil without depending on irrigation. In the present study, two varieties of chickpea, namely ICC 4958 and HC-6, were grown under three field capacities (FC) (100% FC, 50% FC and 25% FC). Samples were taken three times, i.e., 15, 30 and 45 days after sowing (DAS). Parameters such as morphological, physiological, biochemical, metabolite and protein profiling of the two varieties were completed. Morphological parameters such as shoot length (14.2%), number of branches (20.7%), number of leaves (17.5%) and yield (56%) declined as the drought level increased and other characteristics such as root length (...
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A variety of environmental issues are affecting crops all across the world, but rising temperatures are posing the greatest threat. High temperature has been found to drastically inhibit seedling emergence and cause leaf necrosis at the... more
A variety of environmental issues are affecting crops all across the world, but rising temperatures are posing the greatest threat. High temperature has been found to drastically inhibit seedling emergence and cause leaf necrosis at the seedling stage, which results in poor plant stand and significantly decreased yields. Polyamines (PAs) are positively charged, low-molecular-weight aliphatic nitrogenous bases present in all living organisms and are involved in various biological processes in plant growth and development, including senescence and response to different abiotic stresses. Putrescine (Put) functions as a master growth regulator that promotes optimal plant development and greater stress tolerance. Here, the current study aimed to elucidate how Put (1 mM) functions in reducing the negative impacts of high temperature on four varieties of Brassica juncea (RH-1707, RH-1708, RH-1566 and RH-1999-42). Exposure of plants to high temperature resulted in decrease in growth paramet...
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Table S1. Highly differentially expressed proteins (>60 fold) in shoot and root tissues. (XLSX 18 kb)
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Grain yield is one of the most important aims for combating the needs of the growing world population. The role of development and nutrient transfer in flag leaf for higher yields at the grain level is well known. It is a great challenge... more
Grain yield is one of the most important aims for combating the needs of the growing world population. The role of development and nutrient transfer in flag leaf for higher yields at the grain level is well known. It is a great challenge to properly exploit this knowledge because all the processes, starting from the emergence of the flag leaf to the grain filling stages of wheat (Triticum aestivum L.), are very complex biochemical and physiological processes to address. This study was conducted with the primary goal of functionally and structurally annotating the candidate genes associated with different developmental stages of flag leaf in a comprehensive manner using a plethora of in silico tools. Flag leaf-associated genes were analyzed for their structural and functional impacts using a set of bioinformatics tools and algorithms. The results revealed the association of 17 candidate genes with different stages of flag leaf development in wheat crop. Of these 17 candidate genes, t...
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Table S3. Segregation analysis of transgenic lines over-expressing OsHBP1b. (DOCX 15 kb)
Table S1. List of primers used in this study and their sequences (5â ˛ to 3â ˛). (DOCX 16 kb)
Figure S1. Confirmation and molecular analysis of transgenic rice lines over-expressing OsHBP1b. (a) Tissue PCR analysis showed eight positive rice lines over-expressing OsHBP1b. Vector specific forward and gene specific reverse primer... more
Figure S1. Confirmation and molecular analysis of transgenic rice lines over-expressing OsHBP1b. (a) Tissue PCR analysis showed eight positive rice lines over-expressing OsHBP1b. Vector specific forward and gene specific reverse primer pair (F1R1) has been used here. (b) Southern blot analysis showed pattern of digestion (left) and copy number (right) of transgene in the OsHBP1b over-expressing plants. Two lines (L2 and L7) showed intense and clear band having a single copy of transgene and hence, further experimental analysis were done by using L2 and L7 lines. (c) Fold change in expression of OsHBP1b in OsHBP1b over-expressing plant as compared with WT, analyzed through quantitative real-time PCR. Expression of eEF was taken as reference and fold change was calculated using 2-ΔΔCT method. (d) Western blot analysis showed higher OsHBP1b protein accumulation in the OsHBP1b over-expressing plants as compared with the WT. Error bars show standard error from triplicate experiments. Sin...
Table S3. List of RTPCR primers and their sequences (5'to 3') used in this study. (XLSX 10 kb)
Research Interests: Evolutionary Biology, Inorganic Chemistry, Proteomics, Ecology, Medicine, and 4 moreSalinity, Rice, Seedlings, and iTRAQ
Figure S2. DAB staining of the roots of Pokkali and IR64 seedlings in response to 2â h of salinity stress. (TIF 10980 kb)
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Table S2. Differentially expressed proteins in shoot tissues of Pokkali w.r.t IR64 which are commonly appearing under non stress and stress conditions. (XLSX 13 kb)
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Figure S4. Assessment of ABA responsiveness of OsHBP1b over-expressing rice seedlings through morphological and physiological analysis. (a) Photograph of WT and OsHBP1b over-expressing plants in response to exogenous ABA (5 uM). Note the... more
Figure S4. Assessment of ABA responsiveness of OsHBP1b over-expressing rice seedlings through morphological and physiological analysis. (a) Photograph of WT and OsHBP1b over-expressing plants in response to exogenous ABA (5 uM). Note the prominent difference in root morphology where OsHBP1b over-expressing plants have longer roots with more secondary branches as compared to the WT in response to ABA. Bar diagrams showing (b) shoot and root length, (c) fresh weight, (d) Photograph of WT and OsHBP1b over-expressing plants in response to exogenous ABA (10 uM). Bar diagrams showing (e) shoot and root length, (f) fresh weight. The data represent means ± SE of three biological replicates. Single asterisk show significant difference at p
Figure S3. Assessment of high-temperature (45 °C) stress tolerance of OsHBP1b over-expressing rice seedlings through morphological, physiological and enzymatic analysis. (a) Photograph of WT and OsHBP1b over-expressing plants after 12 h... more
Figure S3. Assessment of high-temperature (45 °C) stress tolerance of OsHBP1b over-expressing rice seedlings through morphological, physiological and enzymatic analysis. (a) Photograph of WT and OsHBP1b over-expressing plants after 12 h of high-temperature (45 °C) conditions and subsequent recovery for 3 days. Note the prominent difference in root morphology where OsHBP1b over-expressing plants have longer roots with more secondary branches as compared to the WT under high temperature stress. Bar diagrams showing (b) shoot and root length, (c) fresh weight, (d) Fv/Fm, (e) CAT activity, (f) APX activity and (g) SOD activity measured in leaves of seedlings after 3 days of recovery. The data represent means ± SE of three biological replicates. Single asterisk show significant difference at p
Figure S2. Assessment of drought stress tolerance of OsHBP1b over-expressing rice seedlings through morphological, physiological and enzymatic analysis. (a) Photograph of WT and OsHBP1b over-expressing plants under drought conditions.... more
Figure S2. Assessment of drought stress tolerance of OsHBP1b over-expressing rice seedlings through morphological, physiological and enzymatic analysis. (a) Photograph of WT and OsHBP1b over-expressing plants under drought conditions. Note the prominent difference in root morphology where OsHBP1b over-expressing plants have longer roots with more secondary branches as compared to the WT under drought stress. Bar diagrams showing (b) shoot and root length, (c) fresh weight, (d) Fv/Fm, (e) CAT activity, (f) APX activity and (g) SOD activity measured in the leaves of seedlings after 3 days of drought stress. The data represent means ± SE of three biological replicates. Single asterisk show significant difference at p
Figure S3. Depiction of differentially expressed proteins on metabolic pathways using Mapman. Individual elements in the metabolic overview, stress response and redox overview are indicated by solid red rectangular boxes. Rectangular... more
Figure S3. Depiction of differentially expressed proteins on metabolic pathways using Mapman. Individual elements in the metabolic overview, stress response and redox overview are indicated by solid red rectangular boxes. Rectangular boxes indicate over-represented Mapman functional groups under control and stress conditions in Pokkali with respect to IR64. (TIFF 1525 kb)
ABSTRACT Drought, soil salinity and extreme temperature are major abiotic stresses, which adversely affect the morphological, physiological, biochemical and molecular status of crops. Sustainability of yield and productivity of crops is... more
ABSTRACT Drought, soil salinity and extreme temperature are major abiotic stresses, which adversely affect the morphological, physiological, biochemical and molecular status of crops. Sustainability of yield and productivity of crops is facing serious threat day by day due to the harmful effect of abiotic stresses. Steps are being taken to develop abiotic stress tolerant crops by adopting advanced techniques such as genetic engineering and mutation breeding. In recent era, transgenic crops developed by genetic engineering techniques exhibit tolerance to a range of abiotic and biotic stresses, but issues concerning human nutrition, health, wild life security, effect on non-targeted crops and environmental safety have been raised. Therefore, it is proposed to upgrade the conventional breeding techniques to obtain better stress tolerance and enhanced productivity in crop plants. Mutation breeding is rising up against transgenic plant research in a big way since it does not pose any ethical issues concerning human health and sustenance. Till date, many superior yielding and abiotic stress tolerant crops, including some important varieties of rice, wheat, cotton, sugarcane, potato, corn and soybean have been bred successfully by mutation breeding and are being used for human consumption in many countries. But, certainly as with other techniques, mutation breeding has also some limitations like beneficial mutant frequency is low and it is difficult to control the direction and nature of variation. Hence, improving the mutagenic effectiveness, rapid identification and screening of mutants and exploring the directed mutagenesis approaches are some of the important challenges in this area of research. This chapter presents developments in the area of mutation breeding pertaining to development of crop plants with improved tolerance to abiotic stresses and having improved nutrition.
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Understanding the mode of gene action that controls seed yield and Sclerotinia stem rot resistance in Indian mustard is critical for boosting yield potential. In a line × tester mating design, ten susceptible lines and four resistant... more
Understanding the mode of gene action that controls seed yield and Sclerotinia stem rot resistance in Indian mustard is critical for boosting yield potential. In a line × tester mating design, ten susceptible lines and four resistant testers were used to conduct genetic analysis. The significance of general combining ability (GCA) and specific combining ability (SCA) variances revealed that both additive and non-additive gene actions were involved in the inheritance of Sclerotinia stem rot resistance and yield attributing traits. In addition to 1000-seed weight and number of primary and secondary branches/plant, the genotypes RH 1569 (line) and DRMR 2035 (tester) appeared to be the strongest general combiners for Sclerotinia stem rot resistance. RH 1657 × EC 597317 was the only cross among several that demonstrated a significant desired SCA value for Sclerotinia rot resistance. Regarding SCA effects for yield and component traits, the cross RH 1658 × EC 597328 performed best, with a...
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Figure S1. Evan blue staining of the roots of Pokkali and IR64 seedlings in response to 2â h of salinity stress. (TIFF 314 kb)
Plants, in general, are put to various kinds of stress, biotic and abiotic, both natural and manmade. Infestation by insect pests and diseases, and extreme conditions such as salinity, temperature, etc., as well as heavy metal... more
Plants, in general, are put to various kinds of stress, biotic and abiotic, both natural and manmade. Infestation by insect pests and diseases, and extreme conditions such as salinity, temperature, etc., as well as heavy metal contamination affect their growth performance. Here, we studied the impact of salinity and heavy metal pollution on the growth performance of Indian Mustard Brassica juncea L. and its amelioration by the diamine, putrescine, a known media supplement. We evaluated the putrescine (Put) modulation potential on multiple stress effect in 7-day old Indian mustard. The germination, seedlings length and photosynthetic pigments decline under salinity and metal (Cd/Pb) stress condition, alone or in combination, were checked by putrescine. The stress induced increase in root-shoot ratio, RNA and total amino acids content, as well as Na⁺/K⁺ ratio in leaf tissues were also comparatively less. The increased endogenous Cd/Pb accumulation in plants exposed to either metal fur...
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The heavy ion radiations (HIR)-induced mutations may lead to either lethality or survival; depending on irradiation dose, type of species, physiological status and DNA breaks and their repairibility. In present study, the effects of 7Li3+... more
The heavy ion radiations (HIR)-induced mutations may lead to either lethality or survival; depending on irradiation dose, type of species, physiological status and DNA breaks and their repairibility. In present study, the effects of 7Li3+ ion irradiation exposure on seeds were studied to elucidate the change in total oil that contains important fatty acid including glucosinolate in seeds of Brassica juncea cv RH-30 grown in field for three years. The total seed oil and fatty acid content varied in oil seeds of crop depending on irradiation dose and crop cycle. The palmitic acid, a saturated and cholesterol raising fatty acid decreased at two lower dosage up to M2 generation, but little increased in M3 generation seeds. The unsaturated fatty acids (Oleic, Linoleic, Linolenic and Eicosenoic acid) increased 10-18% depending on dosages over control. There was considerable increase in glucosinolates due to irradiation only in M1 cycle, but thereafter decreased up to M3 cycle. Erucic acid...
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Temperature above or below optimum has a negative impact on plant growth and performance, which leads to a great loss in economic yield. Rice (Oryza sativa L.) is one of the major staple food crops around the world and highly sensitive to... more
Temperature above or below optimum has a negative impact on plant growth and performance, which leads to a great loss in economic yield. Rice (Oryza sativa L.) is one of the major staple food crops around the world and highly sensitive to temperature stress during the reproductive stage. Rice productivity in the tropics, subtropics, and temperate areas is in danger due to low (cold stress) and high (heat stress) temperatures. Both low and high temperatures equally affect both vegetative and generative organs of rice and sometimes lead to complete sterility. In response to temperature stress, a significant variability exists among rice germplasms. Stresses evoke cascades of physiological and molecular events and to increase the global production under such environment, we need to understand the mechanism which will help in the mitigation of stress. In response to temperature stress, protection of structural proteins, enzymes, and membranes, and expression of heat shock proteins (HSPs...
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Fungal disease in crop plants from the past two decades has seen to be increasing which is recognized as a serious threat to food security worldwide. It is difficult for plant to survive under these unfavorable conditions which cause an... more
Fungal disease in crop plants from the past two decades has seen to be increasing which is recognized as a serious threat to food security worldwide. It is difficult for plant to survive under these unfavorable conditions which cause an unprecedented number of fungal and fungal-like diseases which are the most common kind of plant disease. Various approaches such as use of chemical pesticides and other synthetic molecules have been used to control the fungal infections in crop plants. Different transgenic plants have been developed by introducing various genes responsible for resistance in opposition to fungal pathogens. Genes of the enzymes responsible for cell wall degradation are frequently applied to generate transgenic plants for fungal resistance. This chapter mainly emphasizes on how transgenic approach helps to confer plant resistance toward fungal diseases.
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ABSTRACT Vicissitudes of environmental factors, both abiotic and biotic have been fore players towards considerable loss of crop yields over the years. Various approaches to overcome these hurdles in achieving the full potential of crop... more
ABSTRACT Vicissitudes of environmental factors, both abiotic and biotic have been fore players towards considerable loss of crop yields over the years. Various approaches to overcome these hurdles in achieving the full potential of crop plants have heralded us into green revolu-tion through spurt of techniques developed by researchers across the globe. These techniques have utilized broadly three basic biologi-cal platforms, viz. genomics, transcriptomics and proteomics. Although there have been concerted efforts to improve plant stress tolerance, limited success has been achieved in engineering abiotic stress tolerance owing to their complex multigenic nature. In this regard, recent developments in analytical techniques which have enabled the analysis of proteomes of plants under stress condition are being considered a real boon. This review deals with the recent developments in the field of abiotic stress biology with special emphasis on salinity stress and the approaches being employed to engineer stress tolerance. Independent use of the aforesaid approaches have helped us better understand the complexities of stress responses and the available information has been tapped to steer our way to-wards improved genotypes for better performance under stress. However, there is no complacency as ever increasing population and shrinking resources not only demand for sustaining the achieved yield potential but also further increment. For this, better under-standing of abiotic stress responses using a combination of several approaches needs to be undertaken which can help us address these issues more effectively.
Research Interests: Plant Biology and Biology
Plants, in general, are put to various kinds of stress, biotic and abiotic, both natural and manmade. Infestation by insect pests and diseases, and extreme conditions such as salinity, temperature, etc., as well as heavy metal... more
Plants, in general, are put to various kinds of stress, biotic and abiotic, both natural and manmade. Infestation by insect pests and diseases, and extreme conditions such as salinity, temperature, etc., as well as heavy metal contamination affect their growth performance. Here, we studied the impact of salinity and heavy metal pollution on the growth performance of Indian Mustard Brassica juncea L. and its amelioration by the diamine, putrescine, a known media supplement. We evaluated the putrescine (Put) modulation potential on multiple stress effect in 7-day old Indian mustard. The germination, seedlings length and photosynthetic pigments decline under salinity and metal (Cd/Pb) stress condition, alone or in combination, were checked by putrescine. The stress induced increase in root-shoot ratio, RNA and total amino acids content, as well as Na⁺/K⁺ ratio in leaf tissues were also comparatively less. The increased endogenous Cd/Pb accumulation in plants exposed to either metal fur...
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Capparis is a dominating genus of the family Capparidaceae. Capparis spp. are xerophytic, growing in a broad range of climatic conditions, such as dry deserts to cooler terrains of mountain either as shrubs, trees or creepers. The female... more
Capparis is a dominating genus of the family Capparidaceae. Capparis spp. are xerophytic, growing in a broad range of climatic conditions, such as dry deserts to cooler terrains of mountain either as shrubs, trees or creepers. The female flowers of some of the Capparis species are used as vegetable and fruits are used in pickle production because of their high nutritive ingredients like proteins, carbohydrate, minerals and vitamins. Whole plant or parts are used for curing asthma, rheumatism, diabetes, paralysis, toothache, as antihelmintic, antiallergic, snakebite antidote, etc. Out of the many Capparis species, a few are of specific interest for curing particular ailments, like tuberculosis, cancer, rheumatism or diabetes, which still requires extensive study. Simultaneously, it will be valuable to evaluate utility potential of flowers/fruits in cancer patients due to high titre of spermidin containing alkaloids, which are implicated in tumorogenesis. The review highlights medicin...
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Plants, being sessile, are strongly influenced by abiotic stress such as high salt, drought, high temperature and freezing. These factors cause metabolic toxicity, membrane disorganization, closure of stomata, decreased photosynthetic... more
Plants, being sessile, are strongly influenced by abiotic stress such as high salt, drought, high temperature and freezing. These factors cause metabolic toxicity, membrane disorganization, closure of stomata, decreased photosynthetic activity, generation of reactive oxygen species (ROS) and altered nutrient acquisition. In order to meet the increasing demands for plant-based agricultural commodities, it would be imperative to enhance productivity of crop plants. It is well established that tolerance to abiotic stresses is mediated by a number of biochemical reactions and physiological processes, which essentially means that it is a ‘multigenic’ trait. A large number of stress related genes are expressed in an ‘orchestrated manner’ to bring about this stress response. For this ‘stress-responsive’ unique gene expression network to accrue, transcription factors play a very crucial role. Improvement in stress tolerance through engineering of transcription factors genes is emerging as a...
The cadaverine (Cad), an organic diamine was examined for its response on growth in salinity and metal stressed B. juncea cv RH-30 vis-à-vis compared the response of ammonium nitrate. The Cad (1 mM) application ameliorated the effect... more
The cadaverine (Cad), an organic diamine was examined for its response on growth in salinity and metal stressed B. juncea cv RH-30 vis-à-vis compared the response of ammonium nitrate. The Cad (1 mM) application ameliorated the effect caused by salinity and metal stress on seed germination and plant growth. The plant growth recovery (dry biomass accumulation) was dependent on stress and diamine type. The higher growth recovery potential of Cad under both stresses was due to elevation in photosynthetic pigments, nitrate reductase activity and organic nitrogen as well as soluble protein, It is inferred that growth in stressed seedlings was mediated by Cad through lowering endogenous Cd/Pb and Na+ / K+ level in leaf and shoot tissues.
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Research Interests:
Plants constitute immense and diverse niches for endophytic organisms, and their associations are well reported by many researchers. Certain microorganisms like endophytes prevail in the interior portion of plants, like roots, shoots,... more
Plants constitute immense and diverse niches for endophytic organisms, and their associations are well reported by many researchers. Certain microorganisms like endophytes prevail in the interior portion of plants, like roots, shoots, leaves, and stems, and do not harm the host plant. Fungi pose symbiotic relationship with plants, showing diversity in enrichment of resources and habitats. Even though these plant microbial interactions were reported from ancient years, an understanding of the mechanisms enabling these microorganisms to interact with host plants is still a dilemma. Unrevealing such unknown interaction pathways and signaling would be a crucial step in biotechnology which would probably lead to the production of different unique and novel compounds. Such compound may have the ultimate role in various applications in future biotechnology. Similarly, the potential of many isolated fungal endophytes has also not been studied well. Hence, an attempt has been made to coordin...
The present study reports on cation levels in leaf tissue of the mustard seedlings subjected to metal and salinity stress (Cd /Pb 0.1-2mM and NaCl (50-100mM) for 7 days in controlled conditions. The leaf content of Na + /K + decreased... more
The present study reports on cation levels in leaf tissue of the mustard seedlings subjected to metal and salinity stress (Cd /Pb 0.1-2mM and NaCl (50-100mM) for 7 days in controlled conditions. The leaf content of Na + /K + decreased gradually on increase in Pb or Cd level. But under metal and salinity stress, Na + level rose considerably with concomitant decline in K + level. Exogenously applied putrescine caused considerable decline in Na + level, while increased K + level in leaf of the seedlings under low salinity and metal (Pb/Cd) stress. The considerable Na + accumulation in leaf under high saline and metal stress conditions were reversed up to 35% with putrescine. However, the declining trend in Ca ++ /Mg ++ with increasing level of Pb or Cd along with salinity in external environment of the seedlings changed little but significant with putrescine. IAA application to the plant exposed to metal and salinity stress also checked the increase in Na + level, but K + level change ...
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Omega-3 (also called n-3) long-chain polyunsaturated fatty acids have very essential and critical roles in human health due to their multiple health benefits. These important long-chain fatty acids influence a range of health benefits... more
Omega-3 (also called n-3) long-chain polyunsaturated fatty acids have very essential and critical roles in human health due to their multiple health benefits. These important long-chain fatty acids influence a range of health benefits through their cellular, molecular and physiological actions, particularly with respect to the eicosapentaenoic (EPA; 20:5 n-3) and docosahexaenoic (DHA; 22:6 n-3) acids. Essential fatty acids (EFAs) cannot be produced by the human body, as it can be fulfilled through diet only. Marine fish are the major dietary sources of n-3 long-chain polyunsaturated fatty acids, but the increasing demands of fish oil apply huge pressure on declining marine stocks. Recent development in the field of transgenic plants has, however, generated a good deal of excitement among plant biotechnologist, and plants are being looked upon as a potential source for the production of health beneficiary molecules including multicomponent botanical drugs, plant-derived pharmaceuticals, functional foods, dietary supplements and plant-produced recombinant proteins. Many of these products will not only complement conventional pharmaceuticals in the treatment, prevention and diagnosis of diseases but also add value to agriculture and improve the yield quality. An alternative source to achieve the recommended daily intake of EFAs is the need of the hour today. In this chapter, an attempt has, therefore, been made to discuss the impact of omega-3 fatty acid on human health that is facilitated by its biological actions and the new sustainable source in order to produce these fatty acids by the genetic modifications of the plants and their application for health benefits.
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Sclerotinia stem rot is one of the utmost important disease of mustard, causing considerable losses in seed yield and oil quality. The study of the genetic and proteomic basis of resistance to this disease is imperative for its effective... more
Sclerotinia stem rot is one of the utmost important disease of mustard, causing considerable losses in seed yield and oil quality. The study of the genetic and proteomic basis of resistance to this disease is imperative for its effective utilization in developing resistant cultivars. Therefore, the genetic pattern of Sclerotinia stem rot resistance in Indian mustard was studied using six generations (P1, P2, F1, F2, BC1P1, and BC1P2) developed from the crossing of one resistant (RH 1222-28) and two susceptible (EC 766300 and EC 766123) genotypes. Genetic analysis revealed that resistance was governed by duplicate epistasis. Comparative proteome analysis of resistant and susceptible genotypes indicated that peptidyl-prolyl cis-trans isomerase (A0A078IDN6 PPIase) showed high expression in resistant genotype at the early infection stage while its expression was delayed in susceptible genotypes. This study provides important insight to mustard breeders for designing effective breeding p...
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Plants have evolved a number of molecular strategies and regulatory mechanisms to cope with abiotic stresses. Among the various key factors/regulators, transcription factors (TFs) play critical role(s) towards regulating the gene... more
Plants have evolved a number of molecular strategies and regulatory mechanisms to cope with abiotic stresses. Among the various key factors/regulators, transcription factors (TFs) play critical role(s) towards regulating the gene expression patterns in response to stress conditions. Altering the expression of the key TFs can greatly influence plant stress tolerance. OsHBP1b (accession no. KM096571) is one such TF belonging to bZIP family, localized within the Saltol QTL, whose expression is induced upon salinity treatment in the rice seedlings. qRT-PCR based expression studies for OsHBP1b in seedlings of contrasting genotypes of rice showed its differential regulation in response to salinity stress. A GFP based in vivo study showed that the OsHBP1b protein is nuclear localized and possesses the trans-activation activity. As compared to the WT tobacco plants, the transgenic plants ectopically expressing OsHBP1b showed better survival and favourable osmotic parameters (such as germina...
Research Interests: Plant Biology, Antioxidants, Plant Physiology, Reactive Oxygen Species, Escherichia coli, and 15 moreChlorophyll, Rice, CAT, Droughts, Germination, Histones, Cell nucleus, Genotype, Physiological Stress Markers, Amino Acid Sequence, PLANT PROTEINS, Gene expression profiling, Plant Leaves, Molecular Sequence Data, and APX
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Research Interests: Plant Biology and Planta
Research Interests: Plant Biology and Planta
Research Interests:
Research Interests:
Plants, in general, are put to various kinds of stress, biotic and abiotic, both natural and manmade. Infestation by insect pests and diseases, and extreme conditions such as salinity, temperature, etc., as well as heavy metal... more
Plants, in general, are put to various kinds of stress, biotic and abiotic, both natural and manmade. Infestation by insect pests and diseases, and extreme conditions such as salinity, temperature, etc., as well as heavy metal contamination affect their growth performance. Here, we studied the impact of salinity and heavy metal pollution on the growth performance of Indian Mustard Brassica juncea L. and its amelioration by the diamine, putrescine, a known media supplement. We evaluated the putrescine (Put) modulation potential on multiple stress effect in 7-day old Indian mustard. The germination, seedlings length and photosynthetic pigments decline under salinity and metal (Cd/Pb) stress condition, alone or in combination, were checked by putrescine. The stress induced increase in root-shoot ratio, RNA and total amino acids content, as well as Na⁺/K⁺ ratio in leaf tissues were also comparatively less. The increased endogenous Cd/Pb accumulation in plants exposed to either metal fur...
Research Interests:
High salinity is one of the major problems in crop productivity, affecting seed germination as well as yield. In order to enhance tolerance of crops towards salinity, it is essential to understand the underlying physiological and... more
High salinity is one of the major problems in crop productivity, affecting seed germination as well as yield. In order to enhance tolerance of crops towards salinity, it is essential to understand the underlying physiological and molecular mechanisms. In this endeavor, study of contrasting genotypes of the same species differing in their response towards salinity stress can be very useful. In the present study, we have investigated temporal differences in morphological, physiological and proteome profiles of two contrasting genotypes of rice to understand the basis of salt tolerance. When compared to IR64 rice, Pokkali, the salt-tolerant wild genotype, has enhanced capacity to cope with stress, better growth rate and possesses efficient antioxidant system, as well as better photosynthetic machinery. Our proteome studies revealed a higher and an early abundance of proteins involved in stress tolerance and photosynthesis in Pokkali in comparison with IR64, which, in contrast, showed g...
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The cadaverine (Cad), an organic diamine was examined for its response on growth in salinity and metal stressed B. juncea cv RH-30 vis-à-vis compared the response of ammonium nitrate. The Cad (1 mM) application ameliorated the effect... more
The cadaverine (Cad), an organic diamine was examined for its response on growth in salinity and metal stressed B. juncea cv RH-30 vis-à-vis compared the response of ammonium nitrate. The Cad (1 mM) application ameliorated the effect caused by salinity and metal stress on seed germination and plant growth. The plant growth recovery (dry biomass accumulation) was dependent on stress and diamine type. The higher growth recovery potential of Cad under both stresses was due to elevation in photosynthetic pigments, nitrate reductase activity and organic nitrogen as well as soluble protein, It is inferred that growth in stressed seedlings was mediated by Cad through lowering endogenous Cd/Pb and Na+ / K+ level in leaf and shoot tissues.