Sonia Mann

Functional Significance of miR-4693-5p in Targeting HIF1α and Its Link to Rheumatoid Arthritis Pathogenesis

Non-coding RNA, Apr 10, 2024

Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease that causes joint inflamma... more Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease that causes joint inflammation and destruction with an unknown origin. Our study aims to elucidate the molecular mechanism behind HIF1α overexpression in RA. Dysregulated miRNA expressions are known to influence gene behavior, thereby enhancing cell proliferation, inflammation, and resistance to apoptosis, contributing to RA development. Our earlier finding indicated that exogenous miRNA similar to miR-4693-5p may modulate RA-related targets. However, the specific role of miR-4693-5p and its targets in RA remain unexplored. In this study, we found that miR-4693-5p was significantly reduced in PBMCs of RA patients, with evidence suggesting it targets the 3 ′ UTR of HIF1α, thereby potentially contributing to its overexpression in RA. In vitro overexpression of miR-4693-5p leads to the knockdown of HIF1α, resulting in inhibited expression of Survivin to disrupt apoptosis resistance, inflammation suppression, and a reduction in the total cellular ROS response in SW982 and RAFLS cells. The results were validated using the CIA Rat model. In conclusion, this study provides a crucial foundation for understanding the functional role of miR-4693-5p. These findings improve our understanding and provide novel insights into the molecular mechanisms underlying RA pathogenesis.

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Studies on Some Underutilized Edible Fruits in India

Shodhganga, 2016

Phytochemical, Nutritional And Antioxidant Activity Evaluation Of Seeds Of Jackfruit (Artocarpous Heterolphyllus Lam.)

International journal of pharma and bio sciences, 2011

In the present work, we have investigated nutritional, phytochemical content and antioxidant acti... more In the present work, we have investigated nutritional, phytochemical content and antioxidant activity of seeds of the jackfruit (Artocarpus heterophyllus Lam.), one of the most ancient fruit indigenous to Western Ghats of India. The antioxidant properties were evaluated using free radical scavenging, metal chelating, ferric reducing antioxidant power and reducing power assays. Secondary metabolites including alkaloids, saponins, flavanoids and phenolics content were determined in the jackfruit seeds. Nutritional properties including moisture, fats, carbohydrates, proteins, ash content and metal content in the seeds were also estimated. Polyphenolic content and antioxidant properties of dichloromethane: methanol (1:1) extract of jackfruit seeds was found to be high and well correlated. Results indicated jackfruit seeds to be a good source of nutritional and antioxidant components and hold their potential for value addition and nutraceutical development. RAJINDER K. GUPTA University School of Biotechnology, GGS Indraprastha University, Dwarka, New Delhi

Phytochemical, Nutritional And Antioxidant Activity Evaluation Of Fruits Of Ziziphus Nummularia Burm. F

International journal of pharma and bio sciences, 2011

In the present work, we have investigated nutritional, phytochemical content and antioxidant acti... more In the present work, we have investigated nutritional, phytochemical content and antioxidant activity of seeds of the jackfruit (Artocarpus heterophyllus Lam.), one of the most ancient fruit indigenous to Western Ghats of India. The antioxidant properties were evaluated using free radical scavenging, metal chelating, ferric reducing antioxidant power and reducing power assays. Secondary metabolites including alkaloids, saponins, flavanoids and phenolics content were determined in the jackfruit seeds. Nutritional properties including moisture, fats, carbohydrates, proteins, ash content and metal content in the seeds were also estimated. Polyphenolic content and antioxidant properties of dichloromethane: methanol (1:1) extract of jackfruit seeds was found to be high and well correlated. Results indicated jackfruit seeds to be a good source of nutritional and antioxidant components and hold their potential for value addition and nutraceutical development. RAJINDER K. GUPTA University School of Biotechnology, GGS Indraprastha University, Dwarka, New Delhi

INTERNATIONAL JOURNAL OF PHARMACY & LIFE SCIENCES Value addition scenario of arid foods of desert area and evaluation of their nutritional and phytochemical potential

Desert area is gifted with valuable natural resources particularly arid fruits and vegetables. Ar... more Desert area is gifted with valuable natural resources particularly arid fruits and vegetables. Arid fruits are used by native people as a prime source of food with some traditional value attached to it. Processing of traditionally important arid foods into more functional and convenient product can improve livelihood security of the people worldwide. Thus, value addition prospects have been studied and reported along with its nutritional composition, phytochemicals and antioxidant activities. The effect of various solvents on extraction procedure and their activities were also analyzed. The study demonstrated Kair and Kachri the arid fruits of Rajasthan to be rich in proteins and carbohydrates.

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Antitumor Activity of Choerospondias axillaris Fruit Extract by Regulating the Expression of SNCAIP and SNCA on MDA-MB-231 Cells

Asian Pacific Journal of Cancer Prevention, May 1, 2022

Objective: Cancer is a huge problem of disease globally. Today, the percentage of people die from... more Objective: Cancer is a huge problem of disease globally. Today, the percentage of people die from cancer is more than a combination of various diseases. In females, most common types of malignancies that occur are breast and cervical. The present focus has been shifted on medicinal plants as a form of therapy and there is a constant need to identify new therapeutic agents. Choerospondias axillaris (C. axillaris), an underutilized fruit, has been used in the remedy of various diseases. In the present communication, we evaluated the molecular mechanism of C. axillaris methanol extract in regulating cell death in human breast cancer cells (MDA-MB-231). Methods: Methanol extract of C. axillaris was prepared and compounds were screened by Gas chromatography-mass spectrometry. The effect of fruit extract was determined on MDA-MB-231 cells by MTT ((3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay and to analyse the molecular mechanism of human breast cancer cells after treating with fruit extract, protein profiling study was performed by two-dimensional gel electrophoresis. Results: A total 9 differentially expressed proteins were identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS/MS) analysis. Among 9 identified proteins, synphilin-1 protein was found to be significantly downregulated, validated by western blot and RT-qPCR analysis. Possible interacting partners of synphilin-1 (SNCAIP) were analyzed for their possible role in cancer by the in-silico method. Conclusion: Our data implicate that the presence of bioactive compound(s) in C. axillaris fruits might play an important role in inhibiting the proliferation of breast carcinoma cells and Synphilin-1 protein may play a role of apoptotic function.

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Nutritional and phytochemical evaluation of A. lividus L.syn. Amaranthus blitum subsp. oleraceus (L.) Costea leaves

669-674The determination of the proximate composition of A. lividus showed the extract to be rich... more 669-674The determination of the proximate composition of A. lividus showed the extract to be rich in protein (17.28 ± 0.42 gm/100 gm and dietary fiber (8.35 ± 0.16 gm/100 gm) but very low in fat content (0.69 ± 0.05 gm/100 gm). The phytochemical screening of the various extracts revealed the presence of phenolics ranging between 1.38 to 9.07 µg GAE/mg sample, flavonoid in the range 0.88-5.04 µg RE/mg sample, alkaloids (8.16 %) saponins (29.44 %) and tannins (4.27 %). The bioactive compounds were explored through GC-MS which showed presence of compounds like β-sitosterol, phytol and tetradecane in high amounts. Methanolic soxhlet extract showed highest radical scavenging activity against ABTS (IC50 - 1.61±0.01 mg/ml), DPPH (IC50 - 3.18±0.04 mg/ml), FRAP (161.39±0.64 TE/g extract) and reducing power assays. The plant extracts screened against various bacterial strains showed good to moderate antibacterial activity. Findings of the study justify the traditional usage of A. lividus and ...

RESEARCH ARTICLE International Journal of Pharma and Bio Sciences PHYTOCHEMICAL, NUTRITIONAL AND ANTIOXIDANT ACTIVITY EVALUATION OF SEEDS OF JACKFRUIT (ARTOCARPOUS HETEROLPHYLLUS LAM.)

In the present work, we have investigated nutritional, phytochemical content and antioxidant acti... more In the present work, we have investigated nutritional, phytochemical content and antioxidant activity of seeds of the jackfruit (Artocarpus heterophyllus Lam.), one of the most ancient fruit indigenous to Western Ghats of India. The antioxidant properties were evaluated using free radical scavenging, metal chelating, ferric reducing antioxidant power and reducing power assays. Secondary metabolites including alkaloids, saponins, flavanoids and phenolics content were determined in the jackfruit seeds. Nutritional properties including moisture, fats, carbohydrates, proteins, ash content and metal content in the seeds were also estimated. Polyphenolic content and antioxidant properties of dichloromethane: methanol (1:1) extract of jackfruit seeds was found to be high and well correlated. Results indicated jackfruit seeds to be a good source of nutritional and antioxidant components and hold their potential for value addition and nutraceutical development. RAJINDER K. GUPTA

<span style="font-size:11.0pt;font-family: "Times New Roman","serif";mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: Mangal;mso-ansi-language:EN-GB;mso-fareast-language:EN-US;mso-bidi-language: HI" lang="EN-GB">Evaluation of nutritional and phytochemical profiling of Baccaurea ...

135-142<span style="font-size:11.0pt;font-family: " times="" new="&qu... more 135-142<span style="font-size:11.0pt;font-family: " times="" new="" roman","serif";mso-fareast-font-family:"times="" roman";mso-bidi-font-family:="" mangal;mso-ansi-language:en-gb;mso-fareast-language:en-us;mso-bidi-language:="" hi"="" lang="EN-GB">The underutilized fruits Baccaurea ramiflora (Kusum) from the eastern region of India were investigated for nutritional composition, phytochemicals and bioactive compounds. A detailed nutritional characterization of the fruit demonstrated that it has the potential to act as good source of minerals along with other nutrients, dietary fibres, significant phenolic compounds and possesses antioxidant properties. The maximum phenolic, flavonoid and flavonol content were obtained as 32.78µg GAE, 71.67µg RE and 85.58µg RE respectively, per mg of methanol extract. Antioxidant activities (as IC50 value) of different extracts (as DPPH...

<span style="font-size:15.0pt;font-family:"Times New Roman","serif";mso-fareast-font-family: "Times New Roman";mso-bidi-font-family:Mangal;mso-ansi-language:EN-IN; mso-fareast-language:EN-IN;mso-bidi-language:HI">In vitro<span style="font-size:15.0...

183-188<span style="font-size:9.0pt;font-family: " times="" new="&quo... more 183-188<span style="font-size:9.0pt;font-family: " times="" new="" roman","serif";mso-fareast-font-family:"times="" roman";mso-bidi-font-family:="" mangal;mso-ansi-language:en-us;mso-fareast-language:en-us;mso-bidi-language:="" hi"="" lang="EN-US">Oxidative stress causes an imbalance between systemic manifestation of reactive free radicals and biological systems’ ability to detoxify reactive intermediates, thus causing damage to all components of cells. Natural polyphenols with promising antioxidant and free radical scavenging activities can counter oxidative damage in cells. The present study focuses on the antioxidant, antimicrobial, anticancer activity of the MeOH extract of fresh soh-phie (Myrica esculenta Buch.-Ham. ex D. Don) fruits and their relation to phytoconstituents in vitro. The levels of phenolic, flavonoid and flavonol compounds were found to be 26.21±0....

Estrogen mediated differential protein regulation and signal transduction in rheumatoid arthritis

Journal of Molecular Endocrinology, Mar 1, 2022

An alternative perspective of an underutilized fruit tree Choerospondias axillaris in health promotion and disease prevention: A review

Food Bioscience, 2022

Determination of bioactive phytochemicals, antioxidant and anti-inflammatory activity of Colchicum autumnale L. (Suranjanshireen)

Indian Journal of Natural Products and Resources

In Unani System of Medicine Suranjanshireen (Colchicum autumnale L.) is primarily used for the tr... more In Unani System of Medicine Suranjanshireen (Colchicum autumnale L.) is primarily used for the treatment of arthritis, and it belongs to the family of Liliaceae. The current investigation was conducted to evaluate the phytochemical constituents, anti-oxidant, and anti-inflammatory activity of C. autumnale L. Anti-oxidant activity was done using 2, 2'azinobis (3-ethyl benzothiazoline-6-sulfonic acid), and 2, 2-diphenyl-1-picrylhydrazyl free radical scavenging test. The bioactive compounds of the extracts of C. autumnale were identified by GC-MS and UHPLC-QExactiveOrbitrap. Docking studies were carried out for anti-inflammatory activity.The subjective phytochemicals examination demonstrated the existence of phenols, flavonoids, glycosides, and terpenoids. Whereas the quantitative investigation indicated dichloromethane extract contains the maximum number of phenolic and flavonoids constituents and demonstrated the highest antioxidant activity. GC-MS, and UHPLC-QExactiveOrbitrap investigation of the extracts confirmed the existence of bioactive compounds. Docking analysis revealed that colchicoside (3 demethyl colchicine glucoside) inhibits IL-6 having binding energy-7.1 kcal/mol with an RMSD value of 0.00. Phytochemicals, antioxidant, GC-MS, UHPLC-QExactiveOrbitrap analysis and molecular docking results revealed that the compounds presented in C. autumnale L extracts were accountable for numerous therapeutic uses, for instance, antioxidant, and anti-inflammatory activities.

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Two promising under-utilized grains: A Review

Underutilized grains are obtained from those plant species whose ability to safeguard food securi... more Underutilized grains are obtained from those plant species whose ability to safeguard food security, revenue generation, better human health and ecological sustainability have been under-exploited. There is a dire need to utilize these underutilized grains of plant species as they can contribute appreciably towards the improvement in overall human nourishment, stable environmental services, fighting hunger and offering medicinal and income generation options. In this review, two grains, viz. gorgon nut and job’s tears have been discussed. Both of these grains possess antifungal, antibacterial and antioxidant activity and have high phytochemical content. Growth requirements of these grains are specific and distinct and their high nutritional content indicates their potential application as nutraceutical. The utilization of these species is limited because there is a lack of awareness, relevant capacity within the research community and the land they are grown on is increasingly under...

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Phytochemical, *Utritio*Al A*D A*Tioxida*T Activity Evaluatio* of Fruits of Ziziphusummularia Burm. F

In the present work, we have investigated nutritional, phytochemical content and antioxidant acti... more In the present work, we have investigated nutritional, phytochemical content and antioxidant activity of ripe fruits of Ziziphus nummularia Burm. f., one of the underutilized plants distributed in drier parts of North Western India. Fruits were quantitatively analyzed for their total phenolic, flavonoid and flavonol content colorimetrically. Nutritional properties including moisture, fats, carbohydrates, proteins, ash content and mineral content in the fruits were also estimated. The antioxidant properties were evaluated using free radical scavenging, ferric reducing antioxidant power and metal chelating assays. The effect of using different methods of extraction and different solvents on yield of phytochemicals and antioxidant activity was compared and in most cases, acetone extracted sample showed higher yields and strong antioxidant activity. The study demonstrated that Z. nummularia fruits are good source of nutritional and antioxidant components and thus hold potential for nutr...

Phytochemical, Nutritional And Antioxidant Activity Evaluation Of Seeds Of Jackfruit (Artocarpous Heterolphyllus Lam.)

In the present work, we have investigated nutritional, phytochemical content and antioxidant acti... more In the present work, we have investigated nutritional, phytochemical content and antioxidant activity of seeds of the jackfruit (Artocarpus heterophyllus Lam.), one of the most ancient fruit indigenous to Western Ghats of India. The antioxidant properties were evaluated using free radical scavenging, metal chelating, ferric reducing antioxidant power and reducing power assays. Secondary metabolites including alkaloids, saponins, flavanoids and phenolics content were determined in the jackfruit seeds. Nutritional properties including moisture, fats, carbohydrates, proteins, ash content and metal content in the seeds were also estimated. Polyphenolic content and antioxidant properties of dichloromethane: methanol (1:1) extract of jackfruit seeds was found to be high and well correlated. Results indicated jackfruit seeds to be a good source of nutritional and antioxidant components and hold their potential for value addition and nutraceutical development. RAJINDER K. GUPTA University S...

Nutritional and phytochemical evaluation of A. lividus L.syn . Amaranthus blitum subsp. oleraceus (L.) Costea leaves

The determination of the proximate composition of A. lividus showed the extract to be rich in pro... more The determination of the proximate composition of A. lividus showed the extract to be rich in protein (17.28 ± 0.42 gm/100 gm and dietary fiber (8.35 ± 0.16 gm/100 gm) but very low in fat content (0.69 ± 0.05 gm/100 gm). The phytochemical screening of the various extracts revealed the presence of phenolics ranging between 1.38 to 9.07 µg GAE/mg sample, flavonoid in the range 0.88-5.04 µg RE/mg sample, alkaloids (8.16 %) saponins (29.44 %) and tannins (4.27 %). The bioactive compounds were explored through GC-MS which showed presence of compounds like β-sitosterol, phytol and tetradecane in high amounts. Methanolic soxhlet extract showed highest radical scavenging activity against ABTS (IC50 - 1.61±0.01 mg/ml), DPPH (IC50 - 3.18±0.04 mg/ml), FRAP (161.39±0.64 TE/g extract) and reducing power assays. The plant extracts screened against various bacterial strains showed good to moderate antibacterial activity. Findings of the study justify the traditional usage of A. lividus and provide...

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Evaluation of nutritional and phytochemical profiling of Baccaurea ramiflora Lour. syn . Baccaurea sapida (Roxb.) Mull. Arg. fruits

The underutilized fruits Baccaurea ramiflora (Kusum) from the eastern region of India were invest... more The underutilized fruits Baccaurea ramiflora (Kusum) from the eastern region of India were investigated for nutritional composition, phytochemicals and bioactive compounds. A detailed nutritional characterization of the fruit demonstrated that it has the potential to act as good source of minerals along with other nutrients, dietary fibres, significant phenolic compounds and possesses antioxidant properties. The maximum phenolic, flavonoid and flavonol content were obtained as 32.78μg GAE, 71.67μg RE and 85.58μg RE respectively, per mg of methanol extract. Antioxidant activities (as IC50 value) of different extracts (as DPPH scavenging) ranged between 3.89 to 10.14 mg/mL and ABTS with a trolox equivalent antioxidant concentration (TEAC) recorded between 2.55 to 6.23 mg/mL, respectively. GC-MS screening of MeOH extract showed the presence of secondary metabolites and fatty acids. Analysis of volatile flavor showed lauryl alcohol as a major compound followed by the other monoterpenes ...

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INTERNATIONAL JOURNAL OF PHARMACY & LIFE SCIENCES Value addition scenario of arid foods of desert area and evaluation of their nutritional and phytochemical potential

Desert area is gifted with valuable natural resourc es particularly arid fruits and vegetables. A... more Desert area is gifted with valuable natural resourc es particularly arid fruits and vegetables. Arid fr uits are used by native people as a prime source of food with some t raditional value attached to it. Processing of trad itionally important arid foods into more functional and conve nient product can improve livelihood security of th e people worldwide. Thus, value addition prospects have been studied and reported along with its nutritional co mposition, phytochemicals and antioxidant activities. The effe ct of various solvents on extraction procedure and their activities were also analyzed. The study demonstrated Kair and Kachri the arid fruits of Rajasthan to be rich in proteins and carbohydrates.

Nutraceutical Properties of Prosopis cineraria (L.) Druce Pods: A Component of “Panchkuta”

Prosopis cineraria (L.) Druce is a tree endemic to hot deserts of India, belonging to the family ... more Prosopis cineraria (L.) Druce is a tree endemic to hot deserts of India, belonging to the family Leguminosae. Pods locally called “Sangri” are considered as dry fruit of desert and are one of the main ingredients of quintessential Rajasthani dish The Panchkuta. In the present work, we have attempted to study different characteristics of the pods, to understand its health benefits. Sangri pods were studied for various phytochemicals like alkaloids, saponins, tannins, flavanoids, flavanols and total phenolics. As this plant is found in water stress (or deficient area) so antioxidant potential of pods has also been investigated. Acetone and methanol extracts of Sangri pods are the most potent bioactive extracts. Nutritional analysis shows it as a good source of proteins and minerals like calcium, sodium and potassium. This study demonstrates that P. cineraria pods may be employed as nutraceutical food with rich nutrition, disease prevention and health promoting effects. Keyword: Endemi...

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